FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10342205
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97950.041.152831
GGTATCA43750.037.2833981
ATCAACG139050.029.1493423
TCAACGC140800.029.1053984
CAACGCA143650.028.6005465
TATCAAC144700.028.0840442
AACGCAG153350.026.5438736
CTAGGAC40900.021.844313
ACGCAGA190250.021.2686257
CGCAGAG202600.019.938098
GAATCCG19800.019.68756118
AGAGTAC207050.019.5426111
CGCAATA10350.019.33106836
AGTACAT206800.019.21547913
CAGAGTA218900.018.4217410
GCAGAGT222850.018.141749
CTAGCGG12050.018.03514329
AAAGTCG22250.017.98414859
TAGGACT62850.017.727894
TACCGGT9550.017.699417
Produced by FastQC (version 0.11.5)