FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11131221
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA145600.044.6190531
GGTATCA55450.041.577051
TATCAAC201700.032.2698482
ATCAACG203900.031.7853373
TCAACGC206500.031.384154
CAACGCA209400.030.9126765
AACGCAG222500.029.0921256
ACGCAGA266200.024.329257
CGCAGAG279900.023.1503338
GAATCCG33000.022.57338118
AATCCGG33100.022.50508319
AGAGTAC289000.022.43361511
CAGAGTA302350.021.5683510
AGTACAT261700.020.87327813
TACCGGT16800.020.5273047
GGTCTAG24450.020.4610061
CTAGGAC41200.020.1760943
GCAGAGT328500.019.8723249
GAGTACA279800.019.65858512
CGGATCC38900.019.33261140
Produced by FastQC (version 0.11.5)