FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11131221
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117650.043.3343351
GGTATCA49350.037.4317171
TATCAAC169750.030.5447032
ATCAACG169750.030.5227363
TCAACGC173750.029.9182474
CAACGCA177350.029.5447375
AACGCAG186700.027.9174446
ACGCAGA225700.023.0782497
CGCAGAG241650.021.6121758
GAATCCG33800.021.22670718
AATCCGG34150.021.11025619
AGAGTAC246750.021.03928411
CAGAGTA256250.020.43456310
AGTACAT249200.020.40250413
TACCGGT16900.019.7989657
GAGTACA269150.018.95498512
GCAGAGT279150.018.8450589
TATATCG16850.018.83327760
CTAGGAC43450.018.2600233
CTAGTAC20150.017.632973
Produced by FastQC (version 0.11.5)