FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9783281
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG100070.10228674817783523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97300.040.8435971
GGTATCA43100.040.2692681
ATCAACG140150.028.5740133
TCAACGC143450.028.1090454
CAACGCA146850.027.4115435
TATCAAC153700.026.2584172
AACGCAG153500.026.044376
AAAGTCG26200.024.22440359
AGTCGGA29750.021.5654361
CTAGGAC38650.021.4187373
CGCGAAT4400.021.16718928
AGGTTCG30650.020.93197367
ACGAAAG30050.020.89163256
ACGCAGA191900.020.7788587
GAACGAA30700.020.67444254
AGAACGA31200.020.2325653
TCTAGCG12700.020.0992628
CGCAGAG199200.019.9309028
CGCAATA12700.019.82734736
AAGTCGG32400.019.8015960
Produced by FastQC (version 0.11.5)