FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10336266
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53100.043.5966571
GTATCAA129950.043.2558671
TATCAAC186050.030.3859352
ATCAACG188600.029.7913463
TCAACGC191850.029.321354
CAACGCA197150.028.5822665
AACGCAG212550.026.6571456
CTAGGAC44350.023.0201843
TATATCG19050.022.44815660
TACCGGT20700.022.157497
GTACTAG6850.021.9848841
ACGCAGA259000.021.9030917
AATCCGG24250.021.19039319
CGCAGAG272850.020.7913788
GAATCCG25300.020.17463518
AGAGTAC284150.019.87969611
CGACTAT6950.019.85202431
CTCTAGG55300.019.5061651
CAGAGTA291500.019.4966510
TCGACTA7150.019.29672430
Produced by FastQC (version 0.11.5)