FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10336266
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87650.040.9004361
GGTATCA38100.036.3163951
ATCAACG128000.028.3763033
TATCAAC129450.028.0066532
TCAACGC131750.027.5684954
CAACGCA135100.027.0132065
AACGCAG147150.024.6839086
TACCGGT18700.023.057727
GAATCCG24950.022.81178518
AATCCGG24700.022.76347519
CTAGGAC44250.021.2027933
TATATCG20250.020.61053860
ACGCAGA180850.020.0462047
CGCAGAG191500.018.8412978
CTAGTAC23450.018.3867343
CGGATCC32100.018.26732840
AGTACAT192600.018.15987413
ACGGTCG4250.017.85529349
AGAGTAC201400.017.70909711
CGAAATT10950.017.64034748
Produced by FastQC (version 0.11.5)