FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10879618
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99150.039.9571151
GGTATCA48650.035.5464481
ATCAACG139350.028.2438663
TCAACGC141200.027.9462074
TATCAAC141150.027.6902242
CAACGCA145500.027.28655
AACGCAG154800.025.5580656
GAATCCG28750.023.03556618
AATCCGG29950.021.88236619
ACGCAGA192200.020.4233237
TACCGGT15000.020.2361987
CTAGGAC41000.019.3503783
CGCAGAG202050.019.3253368
CGGTCGA8750.019.31637220
CGGATCC35000.018.52777740
TATATCG14800.018.4116660
AGAGTAC208800.018.32080511
AGTACAT201850.018.14810613
CGCGAAT5150.018.08431228
ATCCGGC37200.017.71038220
Produced by FastQC (version 0.11.5)