FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10177267
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG177320.17423145133167872No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA170990.16801170687572606No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA137570.1351738143452461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85150.041.732361
GGTATCA38950.037.1389431
ATTAATC42250.029.6364645
AAAGTCG42650.029.43837459
AGGTTCG42000.028.66137167
ATCAACG129350.027.7345943
AGTCGGA44950.027.62458261
CGCAATA19700.027.31455436
ACGAAAG44550.027.17646856
TCAACGC133350.026.9293254
AAGTCGG46900.026.84373560
CAACGCA135600.026.8133355
TCTAGCG20500.026.7541928
CTAGCGG20800.026.69972829
AGAACGA45900.026.60247453
GAACGAA47000.025.6861854
AATACGA21050.025.39842639
AACGCAG146300.024.7814186
ATACGAA21850.024.31040640
TATCAAC149300.024.121852
Produced by FastQC (version 0.11.5)