FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9976200
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92200.042.771361
GGTATCA39850.040.14461
ATCAACG134100.029.4262493
TATCAAC135400.029.2481162
TCAACGC136300.029.0769464
CAACGCA137900.028.7154255
AACGCAG148900.026.5245766
GGTCTAG24800.021.8308181
AATCCGG19150.021.61463719
ACGCAGA184800.021.3720197
GAATCCG20250.020.2700718
CGCAGAG199950.019.7011268
AGAGTAC200250.019.49886711
ACGTAAA17700.019.09792166
AGTACAT201950.019.02709413
TACCGGT9800.019.006837
GTCCGCT20050.018.57996412
TCGGATA6500.018.57325448
CAGAGTA210400.018.55858610
CGTAAAC18600.018.35927667
Produced by FastQC (version 0.11.5)