FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9827280
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112720.11470111770500077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53100.044.8280531
GTATCAA141350.044.0418361
ATCAACG197100.032.0767823
TATCAAC199000.031.8583182
TCAACGC200200.031.5965124
CAACGCA203100.031.1072775
AACGCAG214300.029.5613736
GAATCCG26300.026.75050218
AATCCGG27600.025.11565219
ACGCAGA254300.024.9250937
CGCAGAG268750.023.5591498
AAAGTCG19750.022.87584559
ATCCGGC30500.022.854420
AGAGTAC278000.022.76319911
TCTAGCG8550.022.58796928
GAGTACT122800.022.21343412
GCAGAGT294350.021.6039079
CAGAGTA295900.021.43257510
CTAGCGG8900.021.31478729
ACGAAAG20850.021.1724156
Produced by FastQC (version 0.11.5)