FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9827280
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88450.040.548961
GGTATCA39750.035.4591831
ATCAACG120300.029.5910513
TCAACGC123650.028.7608724
CAACGCA126600.028.3367735
TATCAAC126650.028.2999342
AACGCAG134450.026.5285026
CGCAATA11250.022.3837136
CTAGCGG11650.021.91093829
ACGCAGA163100.021.5935847
TCTAGCG12550.020.33973928
CGCAGAG174650.020.1456998
AATACGA13250.020.045839
CTAGGAC40750.019.8077243
AGAGTAC179900.019.4616211
TACCGGT12800.019.1338047
AGTACAT177300.018.9876813
AAAGTCG20750.018.784959
TATATCG12400.018.63781560
GTACATG249850.018.4881361
Produced by FastQC (version 0.11.5)