FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10872944
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA142340.1309121062336015No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG136920.12592725576440014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141100.042.9373861
GGTATCA55900.042.8524321
ATCAACG203100.030.2995833
TCAACGC208450.029.6216244
CAACGCA213550.029.0276765
TATCAAC218800.028.1591682
AACGCAG224550.027.467196
ACGCAGA274950.022.3572167
CGCAGAG286450.021.4355648
ATACCGT36450.021.2933866
AATCCGG24550.020.9349819
ACCGTCG33350.020.6870178
CGTCGTA28950.020.61357310
AGTACAT290050.020.40695413
AGAGTAC298150.020.32716211
GCAGAGT302050.020.1458459
TACCGGT12350.020.1107967
CAGAGTA310000.019.65100510
TCTAGCG17050.019.62452328
GAATCCG26800.019.43470418
Produced by FastQC (version 0.11.5)