FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10559688
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA174610.16535526428432354No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG171220.16214494216116992No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCG122840.1163291945746882No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA121850.11539166687500614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.046.2093431
GTATCAA144150.043.720391
ATCAACG209400.030.1435473
TCAACGC214550.029.4036354
CAACGCA218450.028.8598945
TATCAAC221000.028.6099362
AACGCAG231100.027.3093576
ATTAATC38000.025.32735845
CGCAATA14750.024.79871736
ACGAAAG39900.023.7702956
AAAGTCG39650.023.57234859
CTAGCGG15800.023.3583729
TCTAGCG15850.023.28369128
GAACGAA41350.022.8534554
AGGTTCG41950.022.60826367
GAATCCG25000.022.34846318
AATCCGG25350.022.17595119
ACGCAGA288400.021.8833647
AGTCGGA42900.021.86695961
AATACGA17200.021.46798339
Produced by FastQC (version 0.11.5)