FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10559688
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA158160.14977715250677862No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG152360.1442845659833889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52700.047.2878721
GTATCAA133350.043.048191
ATCAACG196200.029.71313
TCAACGC200600.029.1818794
CAACGCA205000.028.6062875
TATCAAC210150.027.8092692
AACGCAG216400.027.082986
ATTAATC39150.024.5834645
AAAGTCG40950.022.91221259
AGTCGGA41600.022.4709761
GAACGAA42450.022.26532454
ACGAAAG41900.022.06373456
ACGCAGA267350.021.8056437
AGGTTCG41750.021.7292767
GAATCCG24200.021.66587318
AATCCGG24350.021.24918619
CGCAGAG279250.020.8517078
CTAGGAC48150.020.3461553
AGTACAT282250.020.29951713
AGAACGA46250.020.2867953
Produced by FastQC (version 0.11.5)