FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9993828
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105110.1051749139568942No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG104810.10487472868254287No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA101470.10153266596143139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68000.049.7094841
GTATCAA184400.044.3584751
TCAACGC265300.030.9659864
ATCAACG265450.030.8841423
TATCAAC266500.030.8664592
CAACGCA270900.030.2852445
AACGCAG282500.029.0655066
ACGCAGA333300.024.6357277
CGCAGAG346450.023.690458
AATCCGG28200.023.48021719
AGAGTAC360500.022.77705611
GAATCCG29200.022.67644118
GCAGAGT369750.022.2070249
GAGTACT124750.022.11541412
CAGAGTA377300.021.80845610
AAAGTCG22900.020.93425659
AGTACAT262700.020.64972513
ACCGTCG21600.020.5955268
CGGATCC31850.020.57829340
TACCGGT11000.020.3780177
Produced by FastQC (version 0.11.5)