FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9993828
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.043.20431
GTATCAA125350.042.763571
ATCAACG172500.031.2746643
TCAACGC176750.030.7762074
CAACGCA181600.030.1068255
TATCAAC181750.029.6088332
AACGCAG189450.028.950516
ACGCAGA228350.023.7469257
AATCCGG26100.023.6571219
CGCAGAG239900.022.6612598
GTGTACG24950.022.3798141
GAATCCG27000.022.35752118
AGGTTCG25100.022.12493367
AGTACAT241150.021.74154913
AAAGTCG25850.021.61698559
AGAGTAC250700.021.50593811
CGCAATA10450.021.45620736
GCAGAGT255550.021.084399
AGTCGGA26800.020.97910561
ATACCGT22900.020.9378976
Produced by FastQC (version 0.11.5)