FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11084087
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG115840.10451018654039797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA123850.044.0560341
GGTATCA49100.038.1088371
ATCAACG178000.030.560233
TATCAAC182100.029.9502282
TCAACGC184350.029.5633034
CAACGCA189050.028.9746825
AACGCAG200200.027.3615766
ACGCAGA245800.022.2154797
CGCAGAG256950.021.1307418
AGAGTAC264200.020.40717111
AGTACAT266650.019.75288813
CAGAGTA275700.019.63101810
GCAGAGT279200.019.2986189
CTAGGAC61250.018.8661633
GAGTACA284450.018.63891612
CTCTAGG66150.018.4705831
CTAGCGG12400.018.08142529
TATCGTC20950.017.61711763
CGTCGTA15950.017.51785710
TAAACGA24100.017.4615116
Produced by FastQC (version 0.11.5)