FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10376534
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84850.040.7647061
GGTATCA39950.035.2443281
ATCAACG126300.027.5029353
CAACGCA130600.026.9405045
TCAACGC129600.026.8814414
TATCAAC134050.025.8882642
ATTAATC24050.025.2436845
GAACGAA23800.025.07306754
AACGCAG142300.024.5799966
ACGAAAG24750.024.5286556
AAAGTCG24950.024.05529659
AGGTTCG25750.022.6380767
TCTAGCG10850.021.93590428
AGTCGGA28100.021.84970961
CTAGGAC39450.021.6849083
AGAACGA27800.021.46532653
CTAGCGG11350.021.27347229
CGCAATA11400.020.27254336
AATACGA11650.020.13359339
ACGCAGA177350.019.6445037
Produced by FastQC (version 0.11.5)