FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11573160
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA228970.197845705062403No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG210600.18197277148160054No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG181620.15693207386746577No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA152590.13184817284129832No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150580.13011139567758503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81800.046.285231
GTATCAA230950.045.6812321
ATCAACG331700.031.7138653
TCAACGC339950.030.9838684
CAACGCA346950.030.3255165
TATCAAC350250.030.1542242
AACGCAG370700.028.38256
TCTAGCG21350.027.94436628
CTAGCGG22100.026.68750829
AAAGTCG45100.026.07643759
GAACGAA45150.025.89456454
AATACGA23100.025.6958439
ACGAAAG46450.025.09625456
ATTAATC47250.024.96892445
AGTCGGA47950.024.67017261
CGAACTA24900.024.51760929
AGAACGA48150.024.49586153
AAGTCGG50350.023.49423460
ACGCAGA448750.023.3999927
AGGTTCG51200.023.03554267
Produced by FastQC (version 0.11.5)