FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11742992
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108550.044.020611
GGTATCA45650.038.8510171
ATCAACG161800.029.7799133
TATCAAC162200.029.7284952
TCAACGC166350.029.0269574
CAACGCA168850.028.4917745
AACGCAG181800.026.4616816
AATCCGG29900.023.99035319
TATATCG15950.022.05467260
GAATCCG32850.021.94094818
CTAGGAC45300.021.6996213
TATCGTC28100.021.6001363
ACGCAGA228150.021.1311827
ATTAATC20450.021.08726545
CACGCGA8400.020.93765313
TACCGGT18650.020.3399987
CGCAGAG245100.019.655618
ACGAAAG22400.019.24498656
CGCAATA8300.019.1249336
AGAGTAC253650.018.9389211
Produced by FastQC (version 0.11.5)