FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10814883
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA131690.12176738296660261No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG124500.11511913721119313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115250.042.6994441
GGTATCA48250.040.2886281
ATCAACG167400.029.5488343
TCAACGC172150.028.7925624
CAACGCA174100.028.4244025
TATCAAC178950.027.6811052
AACGCAG186800.026.4915396
ATTAATC31550.024.71017545
AGGTTCG31900.024.32428467
AAAGTCG32750.023.90412559
ACGAAAG32000.023.70963956
GAACGAA34000.022.31464654
TCTAGCG14150.022.1792728
AGAACGA34250.022.15176453
AGTCGGA36100.021.78140661
CGCAATA14550.021.58160436
CGCGAAT4200.021.34943428
AATACGA14500.021.18134139
ACGCAGA234800.021.1053667
AATCCGG26400.020.9007719
Produced by FastQC (version 0.11.5)