FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10629154
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226100.21271683522508No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG150790.14186453597341803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78350.048.5613561
GTATCAA217550.046.2088971
TATCAAC300900.033.4839632
ATCAACG310150.032.3844763
TCAACGC315350.031.849124
CAACGCA321650.031.2219285
AACGCAG337200.029.8839786
ACGCAGA401150.025.1457637
CGCAGAG413100.024.4432858
AGAGTAC418450.024.10661511
CAGAGTA436500.023.17264610
GAGTACT196150.023.15535212
GCAGAGT448400.022.5729479
GTACTTT211350.021.621114
TACTTTT214050.021.3161515
AGTACTT220900.020.7641413
ACTTTTT222800.020.6027416
AGTACAT270250.020.50728813
CTAGGAC62600.020.11113
GAGTACA283800.019.5644412
Produced by FastQC (version 0.11.5)