FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10548739
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102300.041.4905781
GGTATCA47650.035.703841
ATCAACG153250.027.978013
TCAACGC155550.027.5420134
CAACGCA158500.027.1823865
TATCAAC161550.026.5420862
AACGCAG169500.025.275646
AATACGA12000.023.85781539
TCTAGCG12750.022.99602928
CGCAATA12550.022.26287736
CTAGCGG13600.021.5584729
ATACGAA13400.021.36520840
ATTAATC23900.021.07197445
TACCGGT15000.020.6968177
CTAGGAC36300.020.6205043
ACGCAGA206900.020.5567727
AAAGTCG25250.020.35412859
ACGAAAG25150.020.29791356
CAATACG14950.019.61181338
GAATCCG23450.019.56369418
Produced by FastQC (version 0.11.5)