FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31305623
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG377770.12067161225317255No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA363760.11619637788393479No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGC355560.11357704013748585No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG344500.1100441284941047No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCT342240.10932221345666879No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATG337230.10772186197987499No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG327290.10454671354088688No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA319960.10220528114070752No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT317330.10136517647324891No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTG313090.10001078719947532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA346650.042.9089971
GGTATCA133600.040.0046271
TCAACGC486300.030.9066624
CAACGCA494200.030.665565
ATCAACG494050.030.4637363
AACGCAG526600.028.7914946
ACGCAGA617100.024.596447
CGCAGAG646900.023.303598
AGAGTAC681200.022.08061811
GTACATG1053950.021.7839811
GCAGAGT696650.021.5991369
TATCAAC705250.021.447482
CAGAGTA706850.021.3330510
TACATGG1041800.021.1871222
AGTACAT720950.020.63350313
TCTAGCG152300.020.49612628
CTAGCGG154150.020.27274729
ACATGGG1097200.020.05563
GAGTACA758950.019.70494812
AATACGA168000.018.70509339
Produced by FastQC (version 0.11.5)