FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10170382
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA144010.14159743458996918No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG142360.13997507664903835No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA114840.11291611268878592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99700.043.394711
GGTATCA43000.038.6668361
ATCAACG149400.028.9266873
TCAACGC151800.028.4909484
CAACGCA155550.027.6905045
TCTAGCG12350.027.36701628
CTAGCGG12950.027.1656529
TATCAAC161150.026.9468862
AATACGA13250.026.30761139
AACGCAG168300.025.6739446
CGCAATA13650.025.2812436
ATTAATC32250.024.81572345
ACGAAAG32500.023.98149756
GAACGAA33550.023.33352354
AAAGTCG34250.023.15938859
CGCGAAT6850.022.65636628
AGGTTCG35200.022.4358267
CAATACG15450.022.33805338
GCGAATT7000.022.17196529
AGAACGA35600.022.08620653
Produced by FastQC (version 0.11.5)