FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10170382
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG126350.12423328838582465No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA124370.12228645885670765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83250.042.2461171
GGTATCA37450.034.692271
ATCAACG124700.028.6859513
TCAACGC124650.028.6682364
CAACGCA127100.028.1981475
ATTAATC30050.027.66479345
TATCAAC135400.026.3693872
AAAGTCG30750.025.7999259
AACGCAG139000.025.784196
ACGAAAG30750.025.5753256
GAACGAA31750.025.20423154
AGGTTCG33350.023.5806367
AGAACGA35200.023.12600953
CGCAATA14850.022.76397336
CGCGAAT7000.022.66751928
AGTCGGA34950.022.60058461
TACCGGT15000.021.6165897
CTAGGAC37050.021.413943
CTAGCGG15650.021.37969829
AATACGA15600.021.00578339
Produced by FastQC (version 0.11.5)