FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27130632
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442950.16326564010746228No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG376540.13878777317093094No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA358440.13211634730809071No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG335010.12348035239282298No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT318860.11752767130526116No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG315330.1162265589684752No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATG299250.11029967897541051No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCT297840.10977997121482463No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACC295480.10891010574320568No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTG292130.10767533907798388No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGC290160.10694922256142061No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA278780.10275470177030892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159850.044.8196871
GTATCAA456850.044.3707241
ATCAACG631400.031.7405623
TCAACGC638150.031.3717584
CAACGCA641000.031.2041035
AACGCAG678300.029.5288566
ACGCAGA795450.025.1626457
TATCAAC808650.025.0736392
CGCAGAG815100.024.5691858
AGAGTAC848050.023.61051411
CAGAGTA871300.023.03194610
GCAGAGT878250.022.8653939
GTACATG1046350.021.5131661
TACATGG1039150.020.9461672
TAACGGC41450.020.88789436
AGTACAT716200.020.65600613
ACATGGG1065750.020.1315923
GAGTACA740700.020.00537112
TCTAGCG119550.019.56507928
CTAGCGG120000.019.46296329
Produced by FastQC (version 0.11.5)