FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27130632
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG331550.12220504115053421No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA318620.11743921040984229No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT284990.10504362743927233No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG283960.10466398276309966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA347550.042.1252941
GGTATCA135650.036.7720071
ATCAACG483050.030.2935713
TCAACGC485300.030.2668554
CAACGCA493100.030.0410965
AACGCAG521200.028.3350986
ACGCAGA615100.024.0088987
CGCAGAG626950.023.3685448
TATCAAC658200.022.4603862
AGAGTAC650850.022.3251611
CAGAGTA674850.021.70500410
GCAGAGT676300.021.5810419
TAACGGC41300.021.38219336
GTACATG1017150.021.1013151
TACATGG1006300.020.554882
AGTACAT704250.020.3874613
GAGTACA727050.019.8382712
ACATGGG1035250.019.8063983
TCTAGCG121900.019.66586328
CTAGCGG122900.019.53402329
Produced by FastQC (version 0.11.5)