FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10482177
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA191480.1826719773955353No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG190510.1817465971047808No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA143620.13701352304964895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50850.044.516171
GTATCAA130950.044.2327161
ATCAACG195700.029.6434043
TCAACGC198150.029.2934474
CAACGCA201000.028.9438295
TATCAAC204150.028.569572
AAAGTCG38550.027.8222159
AGGTTCG39200.027.44819567
ACGAAAG39500.026.97844956
AACGCAG215450.026.9541936
GAACGAA40050.026.78043454
ATTAATC39850.026.74775145
CGCAATA15500.026.71542536
TCTAGCG15800.026.62908628
AGAACGA41550.025.8961453
AATACGA16550.025.8552539
AGTCGGA42400.025.70258761
CTAGCGG16550.025.42536429
AAGTCGG45950.023.491760
ATACGAA18200.022.74433540
Produced by FastQC (version 0.11.5)