FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10482177
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG169090.16131191068420234No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA168440.16069181048936684No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA119300.11381223575980448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99150.041.8429151
GGTATCA41300.038.3364641
AGGTTCG36450.030.18657767
AAAGTCG37000.030.01895759
ATCAACG146550.028.503333
ACGAAAG39100.028.49395456
AGTCGGA40250.027.93799461
TCAACGC150550.027.7914454
CGCAATA17650.027.75101536
GAACGAA40250.027.67997254
TCTAGCG17500.027.59545928
ATTAATC41450.027.4621845
CAACGCA154250.027.2596325
CTAGCGG18450.026.73518429
TATCAAC160900.026.0914822
AAGTCGG43850.025.64421760
AACGCAG165450.025.3933456
AGAACGA44400.025.17057453
CAATACG19800.025.08606538
AATACGA19450.024.82775539
Produced by FastQC (version 0.11.5)