FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10647321
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA121880.11447010942940482No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG111400.10462725788017474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57600.044.6603431
GTATCAA151200.044.5304951
TATCAAC224700.029.9180662
ATCAACG227600.029.5063
TCAACGC231800.028.9855774
CAACGCA236750.028.3914455
AACGCAG252900.026.5917746
CGCGAAT5600.026.4833328
CTAGGAC40000.024.0580733
ACGGTCG3650.023.62289849
CTAGCGG11250.022.99532929
AATCCGG26400.022.9920919
GAATCCG27250.022.40157118
ACGAAAG25750.022.36853456
AATACGA11600.022.01252739
ATTAATC26300.021.90527745
TCGGATA7250.021.8840848
AAAGTCG26450.021.77675459
CGCAATA11600.021.71465136
ACGCAGA311850.021.5761057
Produced by FastQC (version 0.11.5)