FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10250307
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127710.1245913902871397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70350.047.793671
GTATCAA186200.044.3824581
TATCAAC261550.031.7453542
ATCAACG266350.031.1203883
TCAACGC267650.030.955594
CAACGCA272950.030.362265
AACGCAG286600.028.903596
AATCCGG31950.025.69019519
GAATCCG32400.025.54627418
ACGCAGA343400.024.1529277
CGCAGAG356400.023.2525758
GAGTACT127050.022.90998612
CGGATCC36450.022.90261540
AGAGTAC368100.022.41991611
GCAGAGT379400.021.9156769
CAGAGTA382350.021.74658610
ATCCGGC39350.021.04332220
GCGGATC39400.020.84178439
AGTACTT143050.020.56450713
TCGGATA6050.020.52387248
Produced by FastQC (version 0.11.5)