FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10468764
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138060.13187803259295938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64250.046.8624841
GTATCAA178950.045.252641
TATCAAC252350.032.1346742
ATCAACG251300.032.089743
TCAACGC253900.031.760534
CAACGCA258300.031.1614785
AACGCAG275300.029.2995856
ACGCAGA326000.024.7533327
CGCAGAG338950.023.7871388
AATCCGG19000.023.4144519
AGAGTAC351900.022.90229411
GAGTACT154100.022.73697512
CAGAGTA365450.022.13774310
GCAGAGT371600.021.7620839
GTACTTT167550.021.0975214
ACTTTTT168600.021.0071416
GAATCCG21100.020.92075718
AGTACTT170000.020.91468613
ACGAAAG20550.020.81030556
TACTTTT170950.020.61749315
Produced by FastQC (version 0.11.5)