FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10468764
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109100.042.430551
GGTATCA45850.037.566011
ATCAACG153350.030.2988723
TCAACGC157300.029.7349624
CAACGCA161200.029.294725
TATCAAC160600.028.8477882
AACGCAG173300.027.249596
ACGCAGA212550.022.1041167
CTAGCGG10900.021.51894229
TCTAGCG10950.021.1056728
CGCAGAG223300.020.9781998
AATCCGG18150.020.9052119
AGAGTAC231600.020.06207311
AGTACAT229400.019.6521613
CAGAGTA239950.019.46493510
AAAGTCG20350.019.32282459
GAATCCG19600.019.18248218
GCAGAGT247750.018.9218229
CGCAATA12000.018.6842136
AATACGA12400.018.6375839
Produced by FastQC (version 0.11.5)