FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10786416
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG173390.1607484821649749No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA172180.15962670084298622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127810.11849162873006196No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA123190.11420846368246876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69500.048.464871
GTATCAA192800.045.1622771
ATCAACG273300.031.7651273
TCAACGC277750.031.255334
CAACGCA284200.030.5307315
TATCAAC286400.030.4214172
AACGCAG300200.028.9490596
TCTAGCG19350.026.37968828
CTAGCGG20000.025.5237729
AAAGTCG40100.024.59849559
ACGCAGA361900.024.0421497
CGCAATA21300.023.81508836
GAATCCG24500.023.64786118
AGTCGGA41750.023.62633961
AATCCGG24850.023.45367819
ATTAATC43300.023.26422345
AGAACGA42700.023.26137553
ACGAAAG42300.023.23706656
CGCAGAG374550.023.2114168
AATACGA22450.022.90247339
Produced by FastQC (version 0.11.5)