FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10225217
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118110.11550855106546884No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG102510.10025215112794182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66650.046.7314871
GTATCAA178800.044.841211
TATCAAC249900.032.1737172
ATCAACG250850.031.9128063
TCAACGC256550.031.1764264
CAACGCA262950.030.4404265
AACGCAG278050.028.7991456
ACGCAGA332500.024.0936227
CGCAGAG348700.022.954278
AGAGTAC360400.022.17091611
GAATCCG20250.021.6283418
CAGAGTA372300.021.54551710
GCAGAGT373150.021.5056829
TACCGGT21600.021.074867
GAGTACT135450.020.52079612
AGTACAT262050.019.8584513
GTACTTT143550.019.53114114
AATCCGG22650.019.48885219
CTAGGAC48750.019.456383
TCTAGCG9500.019.24057228
Produced by FastQC (version 0.11.5)