FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10569958
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136450.045.002421
GGTATCA52150.042.4512251
ATCAACG194500.031.6183913
TCAACGC197150.031.262784
TATCAAC198000.031.1824512
CAACGCA198900.030.9658365
AACGCAG212500.029.032576
ACGCAGA257800.023.9846557
AATCCGG18100.023.43587319
CGCAGAG274000.022.5789578
AGAGTAC283950.021.72785811
GAGTACT115150.021.084212
GCAGAGT298100.020.8230639
CAGAGTA298250.020.76643810
CGCAATA9750.020.52695836
GAATCCG20750.020.27656218
TATCGTC22800.019.9661263
TACCGGT10200.019.9474757
GACCCGT14250.019.8441756
CTAGCGG10200.019.61105329
Produced by FastQC (version 0.11.5)