FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10344753
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93750.042.363781
GGTATCA41300.036.5375331
TATCAAC136250.029.066312
ATCAACG136600.028.989033
TCAACGC141100.028.1864644
CAACGCA142700.027.8470675
AACGCAG151700.026.0813986
TATCGTC25500.022.45421463
GAATCCG26600.021.26651618
ACGCAGA186400.021.2079187
AATCCGG27800.020.5967919
TCGGATA5850.020.04784648
CGCAGAG198450.019.9724048
AGTACAT198050.019.29741113
AGAGTAC205650.019.00431311
CTAGGAC37100.018.5021293
CAGAGTA217550.018.04431510
ATCCGGC32850.017.6404720
GCAGAGT222950.017.5918839
TACCGGT13700.017.3735227
Produced by FastQC (version 0.11.5)