FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10417372
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123880.11891674790916558No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA123380.11843678040872496No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG122120.11722726230761463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79300.047.6295661
GTATCAA205150.043.1736341
ATCAACG285900.030.9950313
TCAACGC290700.030.4952584
TATCAAC295750.030.1503622
CAACGCA297700.029.7876475
AACGCAG310700.028.5746046
ACGCAGA367950.024.1192577
CGCAGAG382500.023.192658
AGAGTAC388250.022.84916511
CTAGCGG14700.022.76291329
CGTCGTA24900.022.5788210
ACCGTCG26350.022.3834158
AAAGTCG25950.022.33087559
GAGTACT145800.022.07176612
GCAGAGT403950.022.0464929
CAGAGTA406700.021.8720810
ATACCGT30050.021.6935336
TCTAGCG15550.021.29444328
CGCAATA15750.021.0318136
Produced by FastQC (version 0.11.5)