FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10417372
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG109880.10547765789682849No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA109680.10528567089665225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54900.042.4750331
GTATCAA130650.041.3090861
ATCAACG188150.028.6750663
TCAACGC192950.027.9973414
CAACGCA195500.027.6149125
TATCAAC202300.026.8239972
AACGCAG207600.026.0552226
TCTAGCG14200.022.3502228
ACGCAGA251600.021.5399027
CTAGCGG14950.020.76747329
AATCCGG24450.020.74047519
GAATCCG24850.020.68416818
CGCAATA15200.020.6523636
CGCAGAG263550.020.6418638
AGAGTAC268400.020.24315811
AATACGA16000.020.05075539
AAAGTCG28100.020.00999359
AGTACAT269950.019.89652313
CAGAGTA279850.019.46422210
GCAGAGT280950.019.3881079
Produced by FastQC (version 0.11.5)