FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004978201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7361543
Sequences flagged as poor quality0
Sequence length75
%GC32

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA4777636.489984504607254TruSeq Adapter, Index 6 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA976361.3262980328988094RNA PCR Primer, Index 43 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA964051.309575995141236TruSeq Adapter, Index 6 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT914471.2422259844165822No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA533150.7242367530828795TruSeq Adapter, Index 6 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451080.6127519733295044No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC399280.5423862904828513TruSeq Adapter, Index 6 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371210.5042556974808134No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA341870.46439992267925356TruSeq Adapter, Index 6 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA305030.41435606638445227RNA PCR Primer, Index 6 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA268470.3646925651320654RNA PCR Primer, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA249590.3390457679864126TruSeq Adapter, Index 6 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246280.3345494280207288No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA178890.24300611977679135TruSeq Adapter, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG174580.237151368945342TruSeq Adapter, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA162640.2209319432081019TruSeq Adapter, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA153860.20900509580668072TruSeq Adapter, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT153400.2083802268084286TruSeq Adapter, Index 6 (96% over 28bp)
GCTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA143750.19527156195379147RNA PCR Primer, Index 6 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137680.18702600799859484No Hit
ATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA133380.18118484127580317TruSeq Adapter, Index 6 (96% over 28bp)
CAGTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA120170.16324023373904087RNA PCR Primer, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC119160.16186823876461767TruSeq Adapter, Index 6 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107980.14668120528535933No Hit
TTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA106830.145119032789729RNA PCR Primer, Index 43 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA103710.1408807908885406TruSeq Adapter, Index 6 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC102110.1387073335033158RNA PCR Primer, Index 43 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA95700.12999991985375892RNA PCR Primer, Index 43 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA89880.12209396861500368TruSeq Adapter, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA86670.11773346973589639TruSeq Adapter, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC85430.11604904026234718TruSeq Adapter, Index 6 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79330.10776273398117758No Hit
ATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAA78600.10677109404916878TruSeq Adapter, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCTCACAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA78320.10639073900675443TruSeq Adapter, Index 6 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)