FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004978202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7361543
Sequences flagged as poor quality0
Sequence length75
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAACATGCAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAA967251.3139229099116856Illumina Single End PCR Primer 1 (100% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT431120.5856380924488249No Hit
ATACACATCTGACGCTGCCGACGAACATGCAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAA235900.32044912323408287Illumina Single End PCR Primer 1 (100% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185890.25251499583714987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175590.2385233639197652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86550.11757046043200454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)