FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10583348
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA140620.13286910720501677No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG133820.12644391925882056No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA108830.10283135355654939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58750.045.374441
GTATCAA145250.043.4342271
ATCAACG208400.030.1404723
TCAACGC211100.029.7704684
CAACGCA214050.029.3553245
TATCAAC218300.028.9324192
AACGCAG228800.027.5073176
CGCAATA14550.022.76562136
ACGCAGA282850.022.2022617
CGCGAAT4350.021.40644828
CGCAGAG297950.021.1000068
AGAGTAC300400.020.95127711
AAAGTCG34650.020.70285259
TCTAGCG16100.020.56437128
AATACGA16250.020.38446839
CAGAGTA312350.020.2597410
CTAGCGG16250.020.16307629
AGGTTCG35250.020.05647567
ATTAATC35700.020.00178145
CTAGGAC46600.019.985533
Produced by FastQC (version 0.11.5)