FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10583348
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA123320.1165226731654293No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG118450.11192110473925643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117200.043.6856961
GGTATCA47700.039.6376721
ATCAACG167850.030.4148393
TCAACGC172550.029.746034
CAACGCA175750.029.1852115
TATCAAC180400.028.319812
AACGCAG186350.027.3214786
GAACGAA34950.022.89663154
AAAGTCG35300.022.47439659
ACGAAAG35500.022.44473356
AGGTTCG35350.022.24682267
ATTAATC35900.022.1959145
CTAGCGG14850.021.83425329
CGCAATA14700.021.82230236
ACGCAGA235750.021.4064147
AGTCGGA37550.021.31104561
TCTAGCG15150.021.17441228
AAGTCGG38600.021.08877860
CGCGAAT5100.020.96682228
ATACCGT31850.020.2525356
Produced by FastQC (version 0.11.5)