FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10941799
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110520.10100715613584202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64900.045.6715241
GTATCAA173200.044.963661
TATCAAC243450.032.1338582
ATCAACG244450.031.8464643
TCAACGC247850.031.4642544
CAACGCA251850.030.8925195
AACGCAG268200.029.0602876
ACGCAGA329700.023.6603897
CGCAGAG338600.023.0282978
AGAGTAC346700.022.42086211
CAGAGTA360750.021.67172210
GAGTACT130750.021.46994612
GCAGAGT373250.021.0475799
GTACTTT142500.019.796614
CGCGAAT7000.019.70752128
CTAGGAC51350.019.6809863
AGTACAT257950.019.23860213
TACTTTT148000.019.1773415
TACCGGT14350.018.9855617
ACTTTTT151800.018.81086516
Produced by FastQC (version 0.11.5)