FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004978209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13495229
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA9078896.727481245409026RNA PCR Primer, Index 17 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2966532.1982064920869444RNA PCR Primer, Index 28 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA2396951.7761462217499235RNA PCR Primer, Index 17 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA949310.7034411939211999RNA PCR Primer, Index 17 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA851610.6310452382838408RNA PCR Primer, Index 17 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC695310.5152265293163977RNA PCR Primer, Index 17 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA617650.4576802661147876RNA PCR Primer, Index 17 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA531160.3935909498090029RNA PCR Primer, Index 17 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA487410.36117208533475054RNA PCR Primer, Index 17 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG390950.2896949729419189RNA PCR Primer, Index 17 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA346070.2564387755109602RNA PCR Primer, Index 17 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA304070.22531666561567795RNA PCR Primer, Index 17 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA299780.22213776439065983RNA PCR Primer, Index 17 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA290310.21512046961189024RNA PCR Primer, Index 28 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC276250.20470197282313626RNA PCR Primer, Index 28 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT273180.20242709479031443RNA PCR Primer, Index 17 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA262780.19472066757814926RNA PCR Primer, Index 17 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA245400.18184204210243488RNA PCR Primer, Index 28 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC242900.17998953556104902RNA PCR Primer, Index 17 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA229790.17027499125802165RNA PCR Primer, Index 17 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA203790.1510089232276088RNA PCR Primer, Index 17 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA203290.15063842191933163RNA PCR Primer, Index 17 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA168150.12459958997361215RNA PCR Primer, Index 28 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAATA163740.12133176843460752RNA PCR Primer, Index 17 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAA162190.1201832143789483RNA PCR Primer, Index 28 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAA158640.11755265509018038RNA PCR Primer, Index 17 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA155200.11500360608923346RNA PCR Primer, Index 17 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG153670.11386987208590532RNA PCR Primer, Index 17 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA152900.11329930007115847RNA PCR Primer, Index 17 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG146020.10820120206926462RNA PCR Primer, Index 28 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA143780.1065413562081829RNA PCR Primer, Index 28 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142950.1059263240364428No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGAAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC136800.10136915794463361RNA PCR Primer, Index 17 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)