FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13495229
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGATCAACCACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAA1326780.9831474515919663Illumina RNA PCR Primer (96% over 32bp)
ATACACATCTGACGCTGCCGACGATCAACCACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAA244500.18117513974753596Illumina RNA PCR Primer (96% over 32bp)
TTATACACATCTGACGCTGCCGACGATCAACCACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAA183250.1357887294835827Illumina RNA PCR Primer (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA46400.040.28991710
GGCTCGG85200.039.754569
TGGCTCG46650.039.3341878
GGGCTCG45550.035.6650438
GATCGGA17250.034.7916610
CTCGGAG52350.034.1289811
TTGGCTC72650.033.5180937
TTGGCGC35200.033.2175257
TTGCTCG26950.033.1475338
CGGCTCG29850.031.4293088
TCGGCTC35750.030.680427
TGCTCGG42900.030.6325179
TTTGGCG38650.030.520056
CTTGGCT72850.030.017076
TCGGAGA60500.029.64490912
CGGAGAT46350.029.46768413
CGTATCA348750.029.12559956
GCCGTAT349900.029.0990954
CGCCGTA354200.028.98969853
CCGTATC351750.028.93624155
Produced by FastQC (version 0.11.5)