FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9311091
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94250.043.3766941
GGTATCA39600.037.116571
ATCAACG136350.030.281873
TCAACGC139150.029.9204224
CAACGCA142650.029.1624435
TATCAAC142300.029.0904452
AACGCAG152950.027.2217146
AATCCGG19600.021.82285719
ACGCAGA190800.021.6950447
CGCGAAT4850.021.33707428
GAATCCG20550.020.47819518
CGCAGAG204100.020.230828
AAAGTCG19400.019.7360559
AGAGTAC209350.019.60750411
AGTACAT202800.019.57669613
ATTAATC20900.019.3105645
ACGAAAG20550.019.30300956
GCAGAGT214150.019.2491769
AGGTTCG21150.019.0808767
CAGAGTA216600.018.95151110
Produced by FastQC (version 0.11.5)