FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10484749
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA166220.15853503026157328No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG160150.15274566897118855No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA114260.10897733460285984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125000.044.238581
GGTATCA50650.040.31051
ATCAACG184950.029.933113
TCAACGC187500.029.5615444
CAACGCA191650.028.9170025
TATCAAC198050.028.0405372
ATTAATC34350.028.01883345
ACGAAAG34450.027.32952556
GAACGAA34600.027.31058954
AACGCAG208400.026.5928216
AAAGTCG36000.026.5360359
AGGTTCG36650.025.97155267
AGTCGGA39200.024.72173361
AGAACGA39350.024.18916953
TCTAGCG13950.022.99172428
AATCCGG23200.022.89229419
AAGTCGG42750.022.83016260
AACGAAA41250.022.82385855
CTAGCGG14150.022.6685929
GAATCCG23750.022.65257518
Produced by FastQC (version 0.11.5)