FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10484749
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA147230.14042300869577326No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG137290.13094257192041509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109400.040.625351
GGTATCA48300.036.6041831
ATCAACG162600.027.2813113
TCAACGC165250.026.8434334
CAACGCA168100.026.5325975
TCTAGCG16200.026.40276128
CTAGCGG16150.026.05733929
TATCAAC177850.025.0403232
ACGAAAG30750.025.01487556
GAACGAA31250.024.94565654
AAAGTCG32250.024.9208459
CGCAATA17100.024.40798836
AACGCAG183200.024.364286
ATTAATC33350.023.9957845
AATACGA17450.023.91820139
AGGTTCG33300.023.09899167
CAATACG19250.022.04017438
ATACGAA18750.021.89162840
AGTCGGA36500.021.82988561
AGAACGA36650.021.458353
Produced by FastQC (version 0.11.5)